Type:MouseIgG
Applications:IHC
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:H
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:ProteinGPurified-liquid
Immunogen:HybridomaresultingfromthefusionofamousemyelomawithBcellsobtainedfromamouseimmunizedwithpurified,NS0-derived,recombinanthumanmGLUR5(rhmGLUR5;aa21-509;Accession#P41594).
Metabotropicglutamatereceptors(mGluRs)areG-proteincoupledreceptorsactivatedbyglutamate.Basedonsequencesimilarity,transductionmechanismsandagoNISTpotencies,mGluRsaresuBDividedintothreegroups:mGluR1/mGluR5,mGluR2/mGluR3,andmGluR4/mGluR6/mGluR7/mGluR8.mGluRsarewidelydistributedthroughoutthenervoussystemandareexpressedbybothneuronsandglialcells.mGluRshavebeensuggestedtoplayavarietyoffunctionalroles,amongwhichisinvolvementinsynapticplasticityunderlyinglearningandmemoryaswellaschronicpain.
Image:Humanmetabotropicglutamatereceptor5(mGluR5) stainingof paraffin-embeddedhumancerebellumsectionsusing25µg/mLmouseanti-humanmGluR5monoclonalantibody. TissuewasstainedusinHRP-DAB(brown)andcounterstainedwithhematoxylin(blue). ThisantibodyspecificallyrecognizeshumanmGluR5.Thisantibodyhasnotyetbeentestedinotherspecies.
Humanmetabotropicglutamatereceptor5(mGluR5;alsomGluR5b)isa150kDa,7-transmembraneglycoproteinthatbelongstogroupIoftheC-familyofG-proteincoupledreceptors.mGluR5isconstitutivelyexpressedandregulatesneuronalionchannelactivity.HumanmGluR5is1212aainlengthandcontainsanN-terminalextracellulardomain(ECD)of558aminoacids.Thereisonealternatespliceform(mGluR5a)thatshowsa32aadeletionbetweenaa877-908inthecytoplasmictail.Overaa19-509,humanmGluR5is98%aaidenticaltomouse,ratanddogmGluR5.