
Type:MouseIgG
Applications:IHC;WB;FC;E
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:H;M;R
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:ProteinGPurified-liquid
Immunogen:HybridomaresultingfromthefusionofamousemyelomawithBcellsobtainedfromamouseimmunizedwithpurified,E.coli-derivedrecombinanthumanv-aktmurinethymomaviraloncogenehomolog1(rhAkt1;aa2-480;Accession#BC000479).

Akt1,Akt2,andAkt3makeupafamilyofserine/threoninekinases,oftenactivateddownstreamofgrowthfactorreceptorsandPI3-Kinase.EachAktfamilymembercontainsanN-terminalpleckstrinhomology(PH)domain,acentralkinasedomain,andaC-terminalregulatorydomain.
Mainrolesareinhibitionofapoptosisandpromotionofproliferation.AKThasbeenshowntoplayaroleinsuchmetabolicprocessesasglucosetransport,glycogensynthesis,glycolysis,andproteinsynthesis.AktperturbationsarethoughttoplayanimportantroleintumOrigenesis.
Image:rhAkt1,rhAkt2,rhAkt3(all5ng;lowerpanel),andlysatesofhumanMCF-7,MDA-MB-123,andmouseTS1cells(upperpanel)wereresolvedbySDS-PAGE.Followingelectrophoresis,recombinantproteinsandlysatesweretransferredtoanImmobilon-Pmembraneandimmunoblottedwith0.2μg/mLanti-panAkt,asdescribedinProtocolsforImmunoblotting.Afifteensecondexposuretofilmisshown.