
Type:RabbitIgG
Applications:WB
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:H
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:AffinityPurified-liquid
Immunogen:KLHcoupledsyntheticpeptide,MFQIPEFEPSEQEDSSSAERGC(correspondingtoaminoacids1-21ofhumanBad).Cysteinewasaddedtothecarboxyl-terminalforcouplingtoanaffinitymatrix.

BAD(Bcl2-antagoNISTOfCellDeath)isamemberoftheBcl2familyandactstopromoteapoptosisbyformingheterodimerswiththesurvivalproteinsBcl2andBclxL,thuspreventingthemfrombindingwithBAX.Badisfoundontheoutermitochondrialmembraneand,oncephosphorylatedinresponsetogrowthstimuli,translocatestothecytoplasm.ThephosphorylationstatusofBadrepresentsakeycheckpointfordeathorcellsurvival.JNK-inducedphosphorylationofBADserine128promotestheapoptoticroleofBADbyopposingtheinhibitoryeffectofgrowthfactoronBad-mediatedapoptosis.Cdc2-inducedphosphorylationofBadserine128hasaninhibitoryeffectonitsinteractionwith14-3-3proteins.ThelatterinteractioniscriticalforBadphosphorylationatserine155,asitewithintheBH3domainthatleadstothereleaseofBclxLandthepromotionofcellsurvival.
Images:ImmunoblotsofSDS-extractsfromhumanJurkat(3x105),A431(2x105),mouseCTLL2(2.5x105),andL929(2x105)cells.Extractswereblottedwith1.0and2.0μg/mLanti-BAD.Incubationwithanti-Badwasovernightat4°CanddetectionwasbytheECLprocedure(Amersham).Oneandfiveminuteexposuresareshown.Thebandthatco-migrateswithrecombinantBadisindicatedbythearrow.Theidentityoftheotherbandsisunknown.