
Type:ChickenIgY
Applications:ICC;IF;WB
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:H
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:Wholeserum-liquid
Immunogen:HumanCaseinKinase1alphaprotein.

TheCKIfamilyconsistsofseveraldifferentgenes,dividedinalpha,beta,gamma,deltaandepsilon,someofwhichareencodedbymorethanonegene.ThesinglemammalianCK1alphagenecangeneratefourdifferentproteinsbyalteratetranscription.CK1awasraisedagainsttheshortestoftheseproteins,andwillthereforebindtoallCK1isotypes.TheCK1alphaproteinsareinvolvedinthecontrolofproteindegradation,andphosphorylateacidicregionsoftheirsubstratemolecules.Typicallythetargetser/thrresiduescloselyC-terminaltotwoorthreeasparticorglutamicacidresidues.Alternately,phosphorylationonresidues3or4aminoacidsN-terminaltoaser/thrresiduecanalsofavorCKIphosphorylation.ThismeansthatCKIfamilyenzymesoftenphosphorylateoneormoreser/thrresiduesC-terminaltoaphosphorylationsitegeneratedbytheactivityofanotherproteinkinase.
Image:HeLacellsstainedwithchickencaseinkinase1alphaantibody(red)andourpanspecificmousemonoclonalantibodytonuclearporecomplexes39C7-aPanspecificNuclearPoreComplexMarker.Caseinkinase1alphahasaparticulatedistributionbothinthenucleusandthecytoplasm.BlueistheHoeschstDNAstain.
CK1alphais playsaroleinAlzheimer"sand SporADIcInclusionBodyMyositis. Expressionofthegeneleadstoaccumulationoftauphospho-epitopesinbothdiseases.ItisalsosUSPectedtobeanapoptosissuppressorbybindingtoMDM2anddownregulatingp53.