
Type:MouseIgM
Applications:IHC;WB
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:B;Ch;H;M;P;R
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:AscitesFluid-liquid
Immunogen:ExtensivelypurifiedpigGAPDH.

GAPDHisa146kDatetramercomposedoffour30-40kDasubunits.Glyceraldehyde3-PhosphateDehydrogenase(GAPDH)isametabolicenzymeresponsIBLeforcatalyzingonestepintheglycolyticpathway,thereversibleoxidativephosphorylationofglyceraldehyde3-phosphate.BecauseGAPDHasaproteinexpressedinlargeamountsandwhichisrequiredatalltimesforanimportanthousekeepingfunctions,levelsofGAPDHmRNAareoftenusedasstandardsinstudiesofmRNAexpression.
Increasingly,scientistsaremakinguseofspecificantibodiestoGAPDHasloADIngcontrolsforwesternblottingexperiments.Apartfromaroleinglycolysis,GAPDHmayhaveotherrolessuchasintheactivationoftranscription.GAPDHisreportedtobindtoavarietyofotherproteins,includingtheamyloidprecursorprotein,mutationsinwhichcausesomeformsofAlzheimer"sdisease,andthepolyglutaminetractsofHuntingtin,theproteinproductaberrantformsofwhicharecausativeofHuntington"sdisease.Associationswithactinandtubulinhavealsobereported.Theproteinmayalsohavearoleintheregulationofapoptosis,andinterestinglymigratesfromthecytoplasmintothenucleuswhencellsbecomeapoptotic.
Image:HumanneuroblastmalineSh-SY5Ystainedwithanti-GAPDH(green)andNF-H(red),counterstainedwithafluorescentDNA probe(blue).