
Type:GoatIgG
Applications:WB
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:H;R
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:AffinityPurified-liquid
Immunogen:Purified,E.coli-derivedrecombinanthumanLivin?,alsoknownasML-IAP(aminoacids1-280),withacarboxyl-terminalsixhistidinetag(GenBankaccession#AF311388).

Theproteinencodedbythisgeneisamemberofthefamilyofinhibitorofapoptosisproteins(IAP)andcontainsasinglecopyofabaculovirusIAPrepeat(BIR)aswellasaRING-typezincfingerdomain.TheBIRdomainisessentialforinhibitoryactivityandinteractswithcaspases,whiletheRINGfingerdomainsometimesenhancesantiapoptoticactivitybutdoesnotinhibitapoptosisalone.Twotranscriptvariantsencodingdifferentisoformshavebeenfoundforthisgene.Thetwoisoformshavedifferentantiapoptoticproperties,withisoformalphaprotectingcellsfromapoptosisinducedbystaurosporineandisoformbprotectingcellsfromapoptosisinducedbyetoposide.
Image:DetectionofLivinαandβ.0.5ngofHistaggedrecombinanthumanLivin-αandhumanLivin-β,andlysatesfrom0.5x106humanSK-Mel-28cellswereresolvedbySDS-PAGE,transferredtoImmobilonmembraneandimmunoblottedwith0.5μg/mLgoatanti-Livin.A60secondexposuretofilmisshown.
RelatedReagentstoConsider:
Survivin
CaspasesandCathepsins
AllApoptosisReagents
DiabetesandObesity Antibodies