
Type:GoatIgG
Applications:IHC;WB;E;NB
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:H
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:AffinityPurified-liquid
Immunogen:Purified,NS0-derived,recombinanthumanmatureSortilin(rhSortilin;aa78-755;Accession#Q99523)extracellulardomain.

NeurotensinReceptor3,also knownasSortilin, isamemberofthefamilyofVps10p-domainreceptors,andisfoundexpressedinbrain,spinalcordandmuscle. NTS3 isexpressedduringembryogenesisinareaswherenervegrowthfactor(NGF)anditsprecursor,proNGF,havewell-characterizedeffects.
Image:Sortilinstainingofimmersionfixedparaffin-embeddedsectionsofhumanbrainusingHumanat1.7µg/mLovernightat4°C.TissuewasstainedusingtheAnti-GoatHRP-DAB (brown)andcounterstainedwithhematoxylin(blue).
ItisrequiredforproteintransportfromtheGolgiapparatustothelysosomesbyapathwaythatisindependentofthemannose-6-phosphatereceptor(M6PR).AlsorequiredforproteintransportfromtheGolgiapparatustotheendosomes.PromotesneuronalapoptosisbymediatingendocytosisoftheproapoptoticprecursorformsofBDNF(proBDNF)andNGFB(proNGFB).Alsoactsasareceptorforneurotensin.MaypromotemineralizationoftheextracellularmatrixduringosteogenicdifferentiationbyscavengingextracellularLPL.ProbablyrequiredinADIpocytesfortheformationofspecializedstoragevesiclescontainingtheglucosetransporterSLC2A4/GLUT4(GLUT4storagevesicles,orGSVs).ThesevesiclesprovideastablepoolofSLC2A4andconferincreasedresponsivenesstoinsulin.MayalsomediatetransportfromtheendoplasmicreticulumtotheGolgi.