
Type:MouseIgG
Applications:IHC;WB
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:H
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:ProteinGPurified-liquid
Immunogen:HybridomaresultingfromthefusionofamousemyelomawithBcellsobtainedfromamouseimmunizedwithpurified,E.coli-derived,recombinanthumanHighTemperatureRequirementProtein-2(rhOmi/HtrA2;aa134-458)withacarboxylterminalsixhistidinetag(GenBankaccession#AF141305).

ThemammalianserineproteaseOmi(alsoknownasHtrA2),wasidentifiedasa49kDaserineprotease,homologoustothebacterialendoproteaseHtrA(high-temperaturerequirement).ItisahomotrimericserineproteasethatlocalizestothemitochondriaandisprocessedtoexposeanN-terminalreaper-likemotifsimilartoSMAC/Diablo.Omi/HTRA2isreleasedfromthemitochondriainresponsetoapoptoticinsultandcaninteractwiththeBIR2orBIR3domainsofXIAPtorelievecaspase-IAPinhibition.
ItissUSPectedtoplayaroleinParkinson"s,Alzheimer"s,Diabetesandcertainformsofcancer.
Image:DetectionofHtrA2/OmiwithMAB1458.LysatesofhumanSK-MEL-28,MCF-7,HEK293andHeLacellswereresolvedbySDS-PAGE,transferredtoImmobilon-Pmembraneandimmunoblottedwith1.0g/mLmonoclonalanti-HtrA2/Omi,asdescribedinProtocolsforImmunoblotting.A45secondexposuretofilmisshown
OtherReagentstoConsider:
Apoptosis
NeurodegenerativeDisease
DiabetesandObesity
Cancer