
Type:MouseIgG
Applications:IHC
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:H
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:Supernatant-liquid
Immunogen:ProkaryoticrecombinantfusionproteincorrespondingtotheextracellularproteinofAPPbetweentheKunitzproteaseinhibitordomainandthebetaamyloidregion.

Alzheimer"sdisease,themostcommoncauseofdementiaintheelderly,existsinbothfamilialandsporADIcforms.Geneticstudieshaveidentifiedthreegenes;beta-amyloidprecursorprotein(APP), Presenilin1 andPresenilin2 which,whenmutated,cancausefamilialformsofAlzheimer"sdisease.APPandAPP-likeproteinsaretransmembraneglycoproteinswithasimilarmodulardomainstructure.
APP-228 hasbeenraisedtotheextracellularportionofAPPbetweentheKunitzproteaseinhibitordomainandthebetaamyloidregion.ThisregionshowstheleasthomologywiththeAPP-likeproteins.APP-228and doesnotcross-reactwithAPP-likeproteins. APP reactswithlargepyramidalcellsaswellassmallerneurons,astrocytesandmicroglia.APP228reactswithlate-stageneurofibrillarytangle-bearingneurons,neuriticprocessessurroundingsenileplaquesandneuropilthreadsingraymatterofAlzheimer"sdiseasebrain.Unmaskingin1mMEDTA(pH8.0)inapressurecookermayberequiredforupto5minutesinorderforthisAPP-228toworkoptimally.
Image:Humancortex,Alzheimer"sdisease:immunohistochemicalstaining usingamyloidprecursorprotein-228.Noteintensestainingofneurofibrillarytanglesandsenileplaques.Paraffinsection
APP228:CustomerPublication