
Type:MouseIgG
Applications:IHC
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:H;R
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:ProteinGPurified-liquid
Immunogen:HybridomaresultingfromthefusionofamousemyelomawithBcellsobtainedfromamouseimmunizedwithpurified,CHO-derived,recombinanthumanbonemorphogeneticprotein7(rhBMP-7).

"WerecentlyorderedyourBMP7antibody,andIuseditonratspinalcordsections.Itworkedbeautifully."MollyFuller(Dr.RobertMillerLab),CaseWesternUniversity.
BMP-7,orosteogenicprotein1(OP-1),isoneofatleast15structurallyandfunctionallyrelatedBMPs,whicharemembersofthetransforminggrowthfactorβ(TGF-β)superfamily.BMPsregulatecartilageandboneformation,embryogenesisandmorphogenesisofvarioustissuesandorgans,andgrowth,differentiation,chemotaxisandapoptosisofvariouscelltypes.BIOLOGicallyactiveBMP-7isadisulfide-linkedhomodimer.CellularresponsestoBMP-7havebeenshowntobemediatedbytheformationofhetero-oligomericcomplexesoftypeIandtypeIIserine/threoninekinasereceptors.
Image:BMP7inparaffin-embeddedhumanprostatecancertissue.HRP-DABreagents(browncolor)wereusedforthedetection.Tissuesectionswerecounterstainedusinghematoxylin(blue).
BMP-7:CustomerPublication