
Type:GoatIgG
Applications:IHC;WB
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:H;M
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:AffinityPurified-liquid
Immunogen:Syntheticpeptide:C-QEQLHQLHSRLIS,fromtheCTerminusoftheproteinsequenceaccordingtoNP_004073.2;NP_075408.1;NP_001128512.1;NP_001128513.1.

Thisgeneencodesthelargestsubunitofdynactin(DCTN),amacromolecularcomplexconsistingof10-11subunitsranginginsizefrom22to150kD.Dynactinbindstobothmicrotubulesandcytoplasmicdynein.Itisinvolvedinadiversearrayofcellularfunctions,includingER-to-Golgitransport,thecentripetalmovementoflysosomesandendosomes,spindleformation,chromosomemovement,nuclearpositioning,andaxonogenesis.Thissubunitinteractswithdyneinintermediatechainbyitsdomainsdirectlybindingtodynein.Alternativesplicingofthisgeneresultsinatleast2functionallydistinctisoforms:aubiquitouslyexpressedoneandabrain-specificone.
Image:DCTN1staining (dilution:2.5µg/ml)stainingofhumanbraintissue,cortex(formalin-fixedparaffin-embedded)followedbybiotinylated horseanti-goatIgGsecondaryantibody, alkalinephosphatase-streptavidinandchromogen.Protocolondata-sheet.
Basedonitscytogeneticlocation,thisgeneisconsideredasacandidategeneforlimb-girdlemusculardystrophy.DefectsinDCTN1arethecauseofPerrysyndrome(PERRYS)alsocalledparkinsonismwithalveolarhypoventilationandmentaldepression.Perrysyndromeisaneuropsychiatricdisordercharacterizedbymentaldepressionnotresponsivetoantidepressantdrugsorelectroconvulsivetherapy,sleepdisturbances,exhaustionandmarkedweightloss.