Type:MouseIgG
Applications:FC
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:H
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:ProteinGPurified-liquid
Immunogen:Clone#:98725
Fibroblastgrowthfactors(FGFs)compriseafamilyofatleasteighteenstructurally-relatedproteinsthatareinvolvedinamultitudeofphysiologicalandpathologicalcellularprocesses,includingcellgrowth,differentiation,angiogenesis,woundhealingandtumOrigenesis.TheBIOLOGicalactivitiesoftheFGFsaremediatedbyafamilyoftypeItransmembranetyrosinekinaseswhichundergodimerizationandautophosphorylationafterligandbinding.Fourdistinctgenesencodingclosely-relatedFGFreceptors,FGFR1-4,areknown.AllfourgenesforFGFRsencodeproteinswithanN-terminalsignalpeptide,threeimmunoglobulin(Ig)-likedomains,anacid-boxregioncontainingarunofacidicresiduesbetweentheIgIandIgIIdomains,atransmembranedomainandthesplittyrosine-kinasedomain.MultipleformsofFGFR1-3aregeneratedbyalternativesplicingofthemRNAs.AfrequentsplicingeventinvolvingFGFR1and2resultsinreceptorscontainingallthreeIgdomains,referredtoastheαisoform,oronlyIgIIandIgIII,referredtoastheβisoform.OnlytheαisoformhasbeenidentifiedforFGFR3andFGFR4.AdditionalsplicingeventsforFGFR1-3,involvingtheC-terminalhalfoftheIgIIIdomainencodedbytwomutuallyexclusivealternativeexons,generateFGFreceptorswithalternativeIgIIIdomains(IIIbandIIIc).AIIIaisoformwhichisasecretedFGFbindingproteincontainingonlytheN-terminalhalfoftheIgIIIdomainplussomeintronsequenceshasalsobeenreportedforFGFR1.MutationsinFGFR1-3havebeenfoundinpatientswithbirthdefectsinvolvingcraniosynostosis.ThecomplexpatternsofexpressionofthesereceptorsaswellasthespecificityoftheirinteractionswiththevariousFGFligandfamilymembersareunderinvestigation(Galzie,Z.etal.,1997,Biochem.CellBiol.75:669-685;Burke,D.etal.,1998,TrendsBiochem.Sci.23:59-62).
Image:HumanU937monocyticcellswerestainedwithAPC-conjugatedanti-humanFGFR2(filledhistogram)orisotypecontrol(openhistogram).
