
Type:RabbitIgG
Applications:IF;WB
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:B;H;M;R
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:AffinityPurified-liquid
Immunogen:C-terminalpeptideofratandmouseGAP43,whichisKEDPEADQEHA,towhichanNterminalCysteineresiduewasaddedtoallowchemicalcouplingtoKeyholeLimpetHemocyanincarrierprotein.
![Picture[2]](images/neuromics/2017/A8x878ax66x1.jpg)
GAP-43(growthassociatedprotein43kDa;alsoknownasneuromodulin)isa24kDa,neurongrowthcone-associatedmemberoftheneuromodulinfamily.Apparentlyduetoitstertiarystructure,itrunsanomalouslyat43kDaonSDS-polyacrylamidegel.RatGAP-43isa226aminoacid(aa)proteinthatcontainsanN-terminallipidbindingmotif,an11aaIQdomain(IQ:Ile-Gln-X-Ser-etc)(aa38-48),andaC-terminuswithfourpotentialserinephosphorylationsites.ThemoleculemayutilizetheIQdomaintobindcalmodulin.ProteinkinaseC-mediatedphosphorylationoftheSerineat41withinthissiteinhibitsthisinteraction.Unphosphorylated,calmodulin-boundGAP-43isalwaysassociatedwithneuriteretraction,whileS41phosphorylatedGAP-43stABIlizesactinfilamentsandpromotesgrowthconeextension.
Image.Mixedneuron-glialculturesstainedwithrabbitantibodytoGAP43(red),blueisDNAstaining.ProtocolandData-sheet
Note:ThisantibodyisusefulforWesternanalysiswhereitrecognizesabandat43kDa.GAP43isaprovenMarkerfordevelopingandregeneratingneurons,andtoalesserextent,reactiveglialcells.Itisusedwidelytospecificallylabelinjuredneuronsandtoscoreneuronalregeneration. Canbeusedwith MouseMonoclonalAntibodyGAP43-Catalog#:MO22118 forduallabelingcellculturesandtissue.