
Type:RabbitIgG
Applications:IF;IHC;WB
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:H;R
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:AffinityPurified-liquid
Immunogen:peptidecorrespondingtotheCterminusofMMP2,specificallyMGPLLVATFWPELPEK,whichwascoupledtokeyholelimpethemocyaninwithglutaraldehyde.

TheImmunogen:Matrixmetalloproteinase-2(MMP2)(gelatinaseA,72kDagelatinase,72kDatypeIVCollagenase),amemberofthematrixmetalloproteinase(MMP)enzymefamily,isinvolvedinthebreakdownofextracellularmatrixinnormalphysiologicalprocesses,suchasembryonicdevelopment,reproduction,andtissueremodeling,aswellasindiseaseprocesses,suchasarthritisandmetastasis.MMP2degradestypeIVcollagen,themajorstructuralcomponentofbasementmembranes.Theenzymeplaysaroleinendometrialmenstrualbreakdown,regulationofvascularizationandtheinflammatoryresponse.MutationsinthisgenehavebeenassociatedwithWinchestersyndrome,Torgsyndromeandnodulosis-arthropathy-osteolysissyndrome.
Image:MMP-2stainingofcross-sectionofinjuredratnerveshowingMMP2expressionbySchwanncellsandotherreactivecells.
MMP2issecretedasaninactiveproenzyme/zymogenwhichisactivatedwhencleavedbyextracellularproteinases.Theproformhasamolecularweightof72kDaandtheactiveform62kDa.AC-terminuspeptide(aa.475-490)wasselectedwhichishighlyconservedanduniquetoMMP2,andispresentinbothpro-andactiveforms.ThepeptidewasconjugatedtoKLHandusetoimmunizerabbits.Thehyperimmuneserumwasaffinitypurifiedonapeptide-coupledsepharosecolumn.Thepurifiedimmunoglobulinrecognizes72kDaand66kDabandscorrespondingtoMMP2onWesternimmunoblots.SometissueextractsalsoshowMMP2complexesanddegradationproducts.PolyclonalMMP2/475antibodyimmunostainsMMP2specificallyinvarioustissuepreparationsincludingparaffinsections.