
Type:MouseIgG
Applications:ICC;IHC;WB;E
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:Ca;Ch;H;M;R
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:Supernatant-liquid
Immunogen:Enzymaticallydephosphorylatedpigneurofilaments;screenedandselectedforNF-Lspecificity

Neurofilamentscanbedefinedastheintermediateor10nmfilamentsfoundinspecificallyinneuronalcells.Intheelectronmicroscopeneurofilamentsappearsas10nmdiameterfibresofindeterminatelengthwhichgenerallyhavefinewispyprotrusionsfromtheirsides.Theyarefoundparticularlyabundantlyinaxonsoflargeprojectionneurons.AntibodiestothevariousneurofilamentsubunitsareveryusefulcelltypeMarkerssincetheproteinsareamongthemostabundantofthenervoussystem,areexpressedonlyinneurons,andarebiochemicallyverystable.
Image:Cellsgrownfromadultratbrain.LargecellinmiddleisstainedwithmousemonoclonaltoNF-LcloneDA2(green).Anothertypeofneuronallineagecellwasstainedwithrabbitpolyclonaltoalpha-internexin(red).Thesecellsweremitoticbuthadseveralcharacteristicsofneurons. Protocolondatasheet.
NF-Lantibodycanalsobeusefulinthediagnosticsofneurofilamentaccumulationsseeninmanyneurologicaldiseases,suchasLouGehrig"sdiseaseandAlzheimer"sdisease.MutationsintheproteincodingregionofthehumanNF-LgenecausesomeformsofCharcot-Marie-Toothdisease.