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Neuromics/Neurofilament alpha-internexin/NF66/MO25017/500 ul

Type:MouseIgG
Applications:ICC;IF;WB
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:H;R
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:Supernatant-liquid
Immunogen:Purifiedrecombinantratalpha-internexinexpressedinandpurifiedfromE.coli.SpecificallyrecognizestheC-terminalnon-helicalextensionofa-internexin.

Alpha-Internexinisa~66kDaClassIVintermediatefilamentsubunitexpressedveryearlyinneuronaldevelopment.Itisrelatedtobutdistinctfromthebetterknownneurofilamenttripletproteins,NF-L,NF-MandNF-H,havingsimilarproteinsequencemotifsandasimilarintronorganization.Manyclassesofmatureneuronscontainalpha-internexininadditiontoNF-L,NF-MandNF-H.Insomematureneuronsalpha-internexinistheonlyneurofilamentsubunitexpressed.Antibodiestointernexinarethereforeuniqueprobestostudyandclassifyneuronaltypesandfollowtheirprocessesinsectionsandintissueculture.Inadditiontheveryearlydevelopmentalexpressionofalpha-internexinmeansitspresenceisanearlyandconvenientdiagnosticfeatureofneuronalProgenitorscellsandothercellcommittedtotheneuronallineage.Theuseofantibodiestothisproteininthestudyofbraintumorshasnotbeenexaminedtodate,butislikelytobeofinterest.
Image:MixedculturesofratCNScellsstainedwith alpha-Internexin(red)andchickenantibodytoMicrotubuleassociatedprotein2(MAP2-green).Thea-internexinislocalizedprimarilyinneuronalaxonsinthesecultures,whiletheperikaryaanddendritesofneuronsstainstronglyforMAP2.