
Type:RabbitIgG
Applications:IHC;WB
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:H;R
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:AffinityPurified-liquid
Immunogen:SyntheticpeptiderepresentingtheCterminalregionofthehumanNOTCHhomolog3(NOTCH1)protein.

TheNotchproteinsitslikeatriggerpenetratingthecellmembrane,withpartofitinsideandpartoutside.Proteinswhenbindingtotheexteriorpartcausethereleaseofaportionoftheinteriorpart,whichtypicallythenmakesitswaytothecellnucleustoaltergeneexpression.TheNotchgenebelongstoafamilyofepidermalgrowthfactor(EGF)likehomeoticgenes,whichencodetransmembraneproteinswithavariablenumberof cysteinerichEGFlikerepeatsintheextracellularregion. Fournotchgeneshavebeendescribedinmammals:Notch1,Notch2,Notch3andNotch4(Int3),whichhavebeenimplicatedinthedifferentiationofthenervoussystemandotherstructures.TheEGFlikeproteinsDeltaandSerratehavebeenidentifiedasligandsofNotch1.
Image:Notch3stainingof ratcerebellum(Bergmannglia-10microncryostatsections).Workingdilutionis1:100-1:300.Stainedtissuewasmountedunderi-BritePlusmountingmedia
Notchsignalingisimportantinembryonicdevelopmentpromotessurvivalandproliferationofstemcells.ThiscouldhavepositiveimplicationsforfunctionalrecoveryfromstrokeandNeurodegenerativediseases.Signalingisoftenrepressedinmanycancers,andfaultyNotchsignallingisimplicatedinmanydiseasesincludingT-ALL(T-cellacutelymphoblasticleukemia),CADASIL(CerebralAutosomalDominantArteriopathywithSub-corticalInfarctsandLeukoencephalopathy),MS(MultipleSclerosis),TetralogyofFallot,Alagillesyndrome,andmyriadotherdiseasestates..
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