
Type:GoatIgG
Applications:IHC;WB;E
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:H;M;R
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:AffinityPurified-liquid
Immunogen:Producedingoatsimmunizedwithpurified,E.coli-derived,recombinantmousePlexinA3(rmPlexinA3;aa34-150;Accession#P70208).

PlexinA3(alsoknownasSEX)isa220kDamemberofthePlexinAsubfamilyofsemaphorinreceptors.MaturemousePlexinA3ispredictedtobean1853aminoacid(aa)typeItransmembraneglycoproteinthatcontainsa1202aaextracellulardomain(ECD)anda629aacytoplasmicregion.TheECDcontainsonesemaphorindomain(aa#34-473),threeplexin-semaphorin-integrin(PSI)domains(aa#492-830),andfourlg-likeplexin-transcriptionfactor(IPT)repeats(aa#841-1209).PlexinA3interactswithneuropilins1and2,andbindsclass3semaphorins.Overaa#35-150,mousePlexinA3is99%and97%aaidenticaltoratandhumanPlexinA3,respectively.
PlexinA3isinvolvedinthedevelopmentofneuralandepithelialtissues.
Image:DetectionofPlexinA3inmouseembryonic(E13.5)spinalcord.Cellswerestainedusing NorthernLightsTM 577Fluorochromelabeledsecondaryantibody(red)andcounterstainedwithDAPI(blue).