
Type:MouseIgG
Applications:ICC;IF;IHC;IP;WB
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:H;R;Rb
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:ProteinGPurified-liquid
Immunogen:AsyntheticpeptidecorrespondingtotheC-terminusofhumanOsteopontin[Swiss-Prot#P10451].

Thematricellularproteinosteopontinbindstocellsurfacereceptorsandissecretedintomanybodyfluidsincludingmilk,bloodandurine,dependingontheorganoforigin.ThismakesosteopontinanidealcandidateforbeingabioMarkerasthesecretedformiseasilyobtainedinthrowawayfluids,andmimicsthecellularenvironmentfromwhichitisreleased.
Image:Osteopontinstainingofhumanlungadenocarcinoma.Inset:WesternblotanalysisofOsteopontinexpressioninU2OSwholecelllysate.ProtocolsonDatasheet.
Osteopontinisimportantinimmuneresponsesandinflammationaswellasbonegenerationandremodeling.Inautisticchildren,serumlevelsofosteopontinarecorrelatedtotheseverityofdisease,probablyduetoabraininflammationpatterninthesechildren.Inaorticvalvesclerosisandstenosis,increasedlevelsofsecretedosteopontinarealsonoted.Osteopontinhasalsobeensuggestedasacancerbiomarker,sinceitisassociatedwithtumorformation,progressionandmetastasis.Inshort,osteopontinisastrongmarkerforbonegrowth,inflammationandcertaincancers.