
Type:MouseIgG
Applications:IHC
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:H
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:ProteinGPurified-liquid
Immunogen:ProkaryoticrecombinantfusionproteincorrespondingtothefullexternaldomainofthehumanCD1amolecule.

CD1aisaproteinof43to49kDexpressedondendriticcellsandcorticalthymocytes.CD1aantigenexpressionhasbeenshowntobeusefulindifferentiatingLangerhanscells,powerfulantigenpresentingcellspresentinskinandepithelia,frominterdigitatingcells.ImmunohistochemicalstudiesforCD1aantigenhavereportedareductioninepidermalLangerhanscellsingraftversushostdiseaseandtheparticipationofCD1aantigen-positivedendriticcellsinatheroscleroticlesionformationandasthmaticinflammation.
CloneMTB1detectscorticalthymocytes,Langerhanscellsinepidermis,interdigitatingcellsofdermisandinterdigitatingcellsofstratifiedsquamousepitheliumoftonsil.CloneMTB1mayalsodetectsmallfocalgroupsoflymphocytesoutsidethegerminalcentersoftonsilindicatingacross-reactionwithCD1bantigen
Image:CD1a(cloneMT1B)stainingofparaffinembeddedhumanskintissue(normal).NoteintensemembranestainingofLangerhanscells.