
Type:GoatIgG
Applications:FC;ICC;IHC;WB
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:H
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:AffinityPurified-lyophilized
Immunogen:MousemyelomacelllineNS0-derivedrecombinanthumanEndoglin.Glu26-Gly256AccessionNumberQ5T9B9

Endoglin(ENG),alsoknownasCD105,isatypeIintegraltransmembraneglycoproteinandisanaccessoryreceptorforTGF-betasuperfamilyligands.Endoglinishighlyexpressedonvascularendothelialcells,chondrocytes,andsyncytiotrophoblastsoftermplacenta.Itisalsofoundonactivatedmonocytes,mesenchymalstemcellsandleukemiccellsoflymphoidandmyeloidlineages.
Image:Endoglin/CD105wasdetectedinimmersionfixedU937humanhistiocyticlymphomacelllineusingGoatAnti-HumanEndoglin/CD105BiotinylatedAntigenAffinity-purifiedPolyclonalAntibody(Catalog#GT15247)at10 µg/mLfor3hoursatroomtemperature.Cellswerestainedusingthe 557‑conjugatedStreptavidinandcounterstainedwithDAPI(blue)
TheroleofendoglinincancercanbecontrADIctingattimessinceitisneededforneoangiogenesisintumors,whichisneededfortumorgrowthandsurvival,yetthereductioninexpressionofendoglinhasinmanycancerscorrelatedwithanegativeoutcomeofthatcancer.