
Type:MouseIgG
Applications:IF;IHC;WB
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:B;H;M;R
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:liquid-Supernatant
Immunogen:FulllengthrecombinantGalectin-3expressedinandpurifiedfromE.coli.

GalectinsareanewfamilyofanimallectinswhichappeartoexhibitavarietyofBIOLOGicalfunctions.Lectins,ofeitherplantoranimalorigin,arecarbohydratebindingproteinsthatinteractwithglycoproteinandglycolipidsonthesurfaceofanimalcells.TheGalectinsarelectinsthatrecognizeandinteractwithbeta-galactosidemoieties.Galectin3isoneofthemoreextensivelystudiedmembersofthisfamilyandisa30kDaprotein.
Image:RatbrainneuralculturesstainedGalectin-3(green)andDNA(blue).Stainingcanbeseeninseveraltypesofgliaandlymphocyticcells,includingthesecellswhichhavethemorphologyofmicroglia.SurroundingcellsrevealnoGalectin-3staining.Protocolondata-sheet.
Galectin3isnormallydistributedinepitheliaofmanyorgans,invariousinflammatorycells,includingmacrophages,aswellasmicroglia,dendriticcellsandKupffercells.Theexpressionofthislectinisup-regulatedduringinflammation,cellproliferation,celldifferentiationandthroughtrans-activationbyviralproteinsmakingitusefulforthestudyofimmuneresponse,diabetes,tumOrigenesisandneurogenesis