
Type:GoatIgG
Applications:IHC;WB;E
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:M
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:AffinityPurified-liquid
Immunogen:NS0-derived,recombinantmouseplateletderivedgrowthfactorreceptoralpha(rmPDGFR)extracellulardomain.

PDGFisoformsexerttheircellulareffectbybindingtothestructurallysimilarreceptortyrosinekinases(RTKs),PDGFRalphaandPDGFRbeta.PDGF-AA,PDGF-BB,PDGF-CC,PDGF-DDandPDGF-ABisofomsbindthetwodistinctcellsurfacePDGFreceptorswithdifferentaffinities.BothPDGFRalphaandPDGFRbetaaremembersoftheclassIIIsubfamilyofreceptortyrosinekinases(RTK).AllclassIIIRTKsarecharacterizedbythepresenceoffiveimmunoglobulin-likedomainsintheirextracellularregionandasplitkinasedomainintheirintracellularregion.PDGFbindinginducesreceptorhomo-andheterodimerizationleADIngtosignaltransduction.Theexpressionofalphaandbetareceptorsareindependentlyregulatedinvariouscelltypes.
Image: PDGF StaininginRαincryostattissuesectionsofmouseembryo(13d.p.c.)using15µg/mLofPDGFRAlpha.Tissueswerestainedusing HRP-DAB(red-brown)andcounterstainedwithhematoxylin(blue).
PDGFhasalsobeenlinkedtoatherosclerosis,fibrosisandmalignantdiseases.
PDGFRAlpha/CD140ACustomerPublications-(forstainingofOligodendrocyteLineage(OL)Cellssee10.1016/j.ajpath.2011.09.018.