
Type:ChickenIgY
Applications:ICC;IF;IHC;WB
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:NotApplicable
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:liquid
Immunogen:C-terminalregionofS.aureus,aminoacids803-1053ofsequenceCCK74173,expressedinandpurifiedfromE.coli.

Image:TransfectedHEK293cellswhichoverexpressingGFP-Cas9-SAfusionproteinwerestainedwithCH22120.CellswhicharetransfectedwithGFP-Cas9arebrightgreen(leftpanel).StainingwithCH22120 isshowninredinmiddlepanel.Inmergedimage(rightpanel),mostHek293cellsarenottransfectedsoonlythenucleusofthesecellscanbevisualizedwithablueDNAstain.RedantibodystainingisonlyseenincellswhichexpressGFP,asexpected,andthesuperimpositionofgreenandredresultsinanorangesignal.
ThediscoveryofCRISPR(ClusteredRegularlyInterspacedShortPalindromicRepeats)haschangedthefieldofgeneediting.TheserepeatedsequencesarefoundinbacterialgenomeswithshortDNAsequencesderivedfromviruseswhichhaveinfectedthebacteriainterspaced.ThesevirallyderivedsequencescanmakeshortRNAsequenceswhichcanhybridizewithspecificviralDNAandtargetanuclease,suchasCas9,totheviralsequence.So,ifthebacteriaareinfectedbythisvirusagain,Cas9canbedirectedtocleavethespecificviralsequenceandsoinactivatethevirus.BycarefuldesignoftheRNAsequencethesystemcanbeusedtospecificallycutDNAvirtuallyanywhere,includinginlivinghumanandothermammaliancells.
OurantibodyisapolyclonalraisedinchickenagainsttheC-terminal251aminoacidsofofthe Staphylococcusaureus proteinandbindsthisproteintransfectedintocells,onwesternblotsandinimmunocytochemistry.Thehomologousregionofthe S.pyogenes isnotcloselyrelatedinaminoacidsequenceand,asexpected,thisantibodydoesnotrecognizethatprotein