
Type:SheepIgG
Applications:ICC;WB;FC;E
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:M
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:AffinityPurified-liquid
Immunogen:Purified,NS0-derived,recombinantmouse5T4extracellulardomain(rm5T4;aa30-361;Accession#AAH58198).
5T4(oncofetalantigen5T4;alsoTPBGandtrophoblastglycoprotein)isa72-80kDaglycoproteinmemberoftheLRRfamilyofproteins.Itisexpressedontrophoblasts,tumorcells,ovariancuboidalepitheliumanddifferentiatingembryonicstemcellsandimpactscelladhesionandmotility.Themouse5T4CDNAencodesatypeItransmembraneproteinprecursorthatis426aminoacids(aa)inlength.Itcontainsa332aaextracellularregion(aa30-361)thatshowsoneSer-richregionfollowedbysevenLeu-richrepeats(aa90-338).Overaa30-361,mouse5T4shares95%and81%aaidentitywithratandhuman5T4,respectively.
Image:5T4stainingofDifferentiatedMouseD3EmbryonicStemCells.MouseD3embryonicstemcellsgrowninthepresenceofserum,withoutLIForafibroblastfeedercelllayerCellewerestainedwith557fluoro-labeled-conjugatedanti-sheepsecondaryantibody(red).NucleiwerecounterstainedwithDAPI(blue).