
Type:GoatIgG
Applications:ICC;WB
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:H;M
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:AffinityPurified-liquid
Immunogen:E.coli-derivedrecombinanthumanBrg1.Gln673-Asn774
![Picture[2]](images/neuromics/2017/A8x92eax66x1.jpg)
Brg1,alsoknownasATP-dependenthelicaseSMARCA4,isa memberoftheSNF2helicasefamilyofmolecules.Itmay facilitateandinhibitgenetranscription.TheencodedproteinispartofthelargeATP-dependentchromatinremodelingcomplexSNF/SWI,whichisrequiredfortranscriptionalactivationofgenesnormallyrepressedbychromatin. Brg1workstoactivateorrepresstranscription. Itisessentialfortheself-renewal/proliferativecapacityofthemultipotentneuralstemcells
Itisalsothoughttobecrucialtothedevelopmentofspermandsmoothmuscleinthegastrointestinaltract.
Image:Brg1wasdetectedinimmersionfixedundifferentiatedratcorticalstemcellsusingGoatAnti-HumanBrg1AntigenAffinity-purifiedPolyclonalAntibodyat10 µg/mLfor3hoursatroomtemperature.Cellswerestainedwith557-conjugatedanti-GoatIgGSecondaryAntibody(red)andcounterstainedwithDAPI(blue).Specificstainingwaslocalizedtonuclei. Protocolanddata-sheet.