
Type:RabbitIgG
Applications:FC;ICC;IF;WB
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:H;M
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:AffinityPurified-liquid
Immunogen:Asyntheticpeptidemadetoaninternalportionofthehumancriptoproteinsequence(betweenresidues100-150).

Cripto-1(CR-1),alsoknownasTeratocarcinoma-derivedgrowthfactor1(TDGF1),isamemberoftheepidermalgrowthfactor(EGF)-GFCfamilyandhasbeenimplicatedinbothembryogenesisandcarcinogenesis.Duringearlyvertebratedevelopment,CR-1functionsasaco-receptorforNodal,atransforminggrowthfactorb(TGFb)familymember,andisessentialformesodermandendodermformationandanterior-posteriorandleft-rightaxisestablishment.Inadulttissues,CR-1isexpressedatalowlevelinallstagesofmammaryglanddevelopment,andexpressionincreasesduringpregnancyandlactation.
Images:Immunocytochemistry/Immunofluorescence:Cripto1antibodywastestedinHeLacellswithDylight488(green).NucleiwerecounterstainedwithDAPI(blue)andtubulinwasstainedwithalphatubulin(red).
Over-expressionofCR-1inmousemammaryepithelialcellsleadstotheirtransformationinvitro,andwheninjectedinmammaryglands,CR-1producesductalhyperplasias.