
Type:MouseIgG
Applications:ICC;IF;IHC;WB
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:R
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:AffinityPurified-liquid

Enolase1orαisalsoknownasnon-neuronalenolase(NNE)andisexpressedinmostkindsoftissue,butisabsentfromneurons.AbnormalexpressionofNNEisassociatedwithtumorprogressioninsomebreastandheadandneckcancer.Enolase2orγisalsoknownasneuronspecificenolase(NSE),(Checkoutour NeuronSpecificEnolase(NSE)).AswitchfromNNEtoNSEoccursinthedevelopmentofneurons.Enolase3orβisexpressedprimarilyinmusclecells.
MonoclonalantibodyMO22153wasraisedagainsttheN-terminal12aminoacidsofbovineenolase1whichwassynthesizedona8-aminelysinecoreusingthemultipleantigenpresentationmethod.Theantibodywastestedforbindingtoexpressedbovineenolase1,2and3andshowntobespecificforonlyenolase1.
Image:NIH/3T3cellsstainedwithMO22153(green)andcounterstainedwithchickenpolyclonalantibodytovimentinCH22108 (red)andDNA(blue).TheMO22153antibodyrevealsstrongcytoplasmicstaining,whilethevimentinantibodyrevealscytoplasmicintermediatefilaments