
Type:GoatIgG
Applications:ICC;WB
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:H
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:AffinityPurified-liquid
Immunogen:NS0-derived,recombinanthumanGATA-1(rhGATA-1).

GATAproteinsaretranscriptionalcoactivatorswithtwoGATA-typezincfingers.GATAsbindtotheconsensusDNAsequence(A/T)GATA(A/G)tocontroldiversetissue-specificprogramsofgeneexpressionandmorphogenesis.GATA-1,-2,-3areselectivelyexpressedinhematopoieticandendothelialcells,whileGATA-4,-5,and-6areexpressedindevelopingheartandgut-derivedtissues.GATA-1and-3existinmultiplealternativelysplicedisoforms.
Image:DetectionofHumanGATA2byWesternBlot.WesternblotshowslysatesofNIH3T3mouseembryonicfibroblastcelllineandKG1humanacutemyelogenousleukemiacellline.PVDFmembranewasprobedwith0.5μg/mLofHumanGATA2AntigenAffinitypurifiedPolyclonalAntibodyfollowedbyHRPconjugatedAntiGoatIgGSecondaryAntibody.AspecificbandwasdetectedforGATA2atapproximately51kDa(asindicated).
GATA2isexpressedinhematopoieticProgenitors,includingearlyerythroidcells,mastcells,andmegakaryocytes,andalsoinnonhematopoieticembryonicstemcells.Inchickenerythroidprogenitors,forcedexpressionofGATA2promotesproliferationattheexpenseofdifferentiation.