
Type:GoatIgG
Applications:IHC;WB;E
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:H;M;R
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:AffinityPurified-liquid
Immunogen:Purified,E.coli-derived,recombinanthumanHairyandEnhancerofSplit-1(rhHES-1;aa93-280;Accession#Q14469).

HES-1(HairyandEnhancerofSplit-1)isatranscriptionalrepressorthatisatargetofnotchsignaling.Itcontainsabasichelix-loop-helix(bHLH)DNA-bindingdomain,anOrangedomainandaC-terminaltetrapeptideWRPWmotifthatbindstotheGroucho(Gro)/TLE/Grgfamilyofcorepressors.HES-1canformbothhomo-andheterodimerswithotherHESfamilymembers.DimerizationismediatedthroughboththebHLHandthedownstreamOrangedomain.Overthesequenceusedforimmunization,humanHES-1shares97%and98%aminoacidsequenceidentitywithmouseandratHES-1,respectively.Hes1deficientembryosshowseveredefectsinneuronaldevelopment,aswellaspancreatichypoplasia.ItisbelievedtoexertinfluenceonthedevelopmentofrADIalGlialCells.HES-1couldplayaroleasatumorsuppressorinepithelialcells,andasamediatorof17beta-estradiolshavearoleinproliferatingbreastcancercells.
Image:HES-1 stainingof paraffin-embeddedhumanovariancancertissue.HRP-DABreagents(browncolor)wereusedforthedetection.Tissuesectionswerecounterstainedusinghematoxylin(blue).
HES-1:CustomerPublications