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Neuromics/High-mobility group protein B1/HMGP1/MO22134/100 ul

Type:MouseIgG
Applications:IF;IHC;WB
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:H;M;P;MultipleMammalian
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:AffinityPurified-liquid
Immunogen:FulllengthrecombinanthumanHMGB1protein

High-mobilitygroupproteinB1orHMGB1isamongthemostimportantchromatinproteins.InthenucleusHMGB1interactswithnucleosomes,transcriptionfactorsandhistones.[3]ThisnuclearproteinorganizestheDNAandregulatestranscription.
Image:HeLacellsgrownintissuecultureandstainedwithHMGB1(red),Neuromics’chickenpolyclonalantibodytoVimentin(Cat#CH22108)(green)andDNA(blue).TheHMGB1antibodyrevealsstrongnuclearstainingwhichoverlapswiththeDNAstain.ProtocolonDatasheet.
Itisreleasedbyactivatedmacrophages,andserumlevelsincreasesignificantlyduringendotoxaemia,sepsisandarthritiswithsignificantdelayedkineticsincomparisonwithtumournecrosisfactor(TNF)andinterleukin-1beta.RecentlyidentifiedBIOLOGicalactivitiesofHMGB1includeactivationofmacrophages/monocytestoreleaseproinflammatorycytokines,upregulationofendothelialadhesionmolecules,stimulationofepithelialcellbarrierfailure,andmediationoffeverandanorexia.Passiveimmunizationwithanti-HMGB1antibodiesconferssignificantprotectionagainstlethalendotoxaemia,sepsis,arthritisandlipopolysaccharide-inducedacutelunginjury.