
Type:MouseIgG
Applications:IHC;WB
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:H
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:ProteinGPurified-liquid
Immunogen:HybridomaresultingfromthefusionofamousemyelomawithBcellsobtainedfromamouseimmunizedwithpurified,NS0-derived,recombinanthumanInsulin-likeGrowthFactorBindingProtein3(rhIGFBP-3).

Thesuperfamilyofinsulin-likegrowthfactor(IGF)bindingproteinsincludethesixhigh-affinityIGFbindingproteins(IGFBP)andseverallow-affinitybindingproteinsreferredtoasIGFBPrelatedproteins(IGFBP-rP).AllIGFBPsuperfamilymembersarecysteine-richproteinswithconservedcysteineresidues,whichareclusteredintheamino-andcarboxy-terminalthirdsofthemolecule.IGFBPsmodulatetheBIOLOGicalactivitiesofIGFproteins.SomeIGFBPsmayalsohaveintrinsicbioactivitythatisindependentoftheirABIlitytobindIGFproteins.Post-translationalmodificationsofIGFBP,includingglycosylation,phosphorylationandproteolysis,havebeenshowntomodifytheaffinitiesofthebindingproteinstoIGF.
Image:IGFBP-3stainingofParaffin-embeddedtissue.Sectionsofhumanplacentawerestainedusinganti-mouseABC-HRP,AEC(red)substrateandHaematoxylin(blue).