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Neuromics/TNF-alpha Human Sandwich ELISA Kit/ELH-TNFalpha-003/5 Plate Kit

Type:Kit
Applications:E
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:H
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;All
Sensitive,SpecificandCostEffective
TNF-alpha(tumornecrosisfactor-a)issecretedbymacrophages,monocytes,neutrophils,T-cells,NK-cellsfollowingtheirstimulationbybacteriallipopolysaccharides.HumanTNF-alphaisanon-glycosylatedproteinof17.5kDaandalengthof157aminoacid.TNF-alphashowsawidespectrumofBIOLOGicalactivities.Itcausescytolysisandcytostasisofmanytumorcellinesinvitro.WithinhoursafterinjectionTNF-alphaleadstothedestructionofsmallbloodvesselswithinmalignanttumors.TNF-alphaalsoenhancesphagocytosisandcytotoxicityinneutrophilicgranulocytesandalsomodulatestheexpressionofmanyotherproteins.Ingeneral,TNF-alphaandTNF-betadisplaysimilarspectraofbiologicalactivitiesinvitrosystemsalthoughTNF-betaisoftenlesspotentordisplaysapparentpartialagoNISTactivity.
Figure:TNF-alphastandardcurve
SuggestedApplication:QuantitativeProteinDetection;EstablishingNormalRange;ValidationofAntibodyArrayResults.
Figure:BoxplotoftheserumIL-6levelintheRApatientsandcontrolaccordingtotheIL-6(−174G/C)promoterpolymorphismtype.http://dx.doi.org/10.1016/j.ejr.2012.11.002