
Type:MouseIgG
Applications:FC
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:H;R
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:ProteinGPurified-liquid
Immunogen:Clone#:165131

TrkA,theproductoftheproto-oncogenetrk,isamemberoftheneurotrophintyrosinekinasereceptorfamily.TrkA,TrkBandTrkCpreferentiallybindNGF,NT-4andBDNF,andNT-3,respectively.AllTrkfamilyproteinsshareaconservedcomplexsubdomainorganizationconsistingofasignalpeptide,twocysteine-richdomains,aclusterofthreeleucine-richmotifs,andtwoimmunoglobulin-likedomainsintheextracellularregion,aswellasanintracellularregionthatcontainsthetyrosinekinasedomain.TwodistinctTrkAisoformsthatdifferbya6aminoacidinsertionintheirextracellulardomainhavebeenidentified.ThelongerTrkAisoformistheonlyisoformexpressedwithinneuronaltissues,whereas,theshorterTrkAisexpressedmainlyinnon-neuronaltissues.NGFbindstoTrkAwithlowaffinityandactivatesitscytoplasmickinase,initiatingasignalingcascadethatmediatesneuronalsurvivalanddifferentiation.HigheraffinitybindingofNGFrequiresthecoexpressionofTrkAwiththep75NGFreceptor(NGFR),amemberofthetumornecrosisfactorreceptorsuperfamily.NGFRbindsallneurotrophinswithlowaffinityandmodulatesTrkactivity,aswellasaltersthespecificityofTrkreceptorsfortheirligands.NGFRcanalsomediatecelldeathwhenexpressedindependentofTrk(Esposito,D.etal.,2001,J.Biol.Chem.276:32687;Sofroniew,M.V.etal.,2001Annu.Rev.Neurosci.24:1217).