
Type:GoatIgG
Applications:IHC;WB;E
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:H
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:AffinityPurified-liquid
Immunogen:Purified,E.coli-derived,recombinanthumanADPN(rhADPN;aa160-349;Accession#Q9NST1).
![Picture[2]](images/neuromics/2017/A8x6963x66x1.jpg)
HumanADPN(ADIponutrin;alsoPNPLA3andpatatin-likephospholipasedomain-containingprotein3)isa53kDamemberofthePNPLAfamilyofphospholipaseA2enzymes.Itisexpressedinwhiteadipocytes.Adiponutriniselevatedinresponsetoinsulinandappearstoactnotasaphospholipase,butamoleculeinvolvedinanabolicpathways.ADPNislikelytobeatypeIItransmembraneproteinthatis481aminoacids(aa)inlength.Itcontainsonepatatindomain(aa10-179)thatisassociatedwithinvitroenzymeactivityandatransmembranesegmentbetweenaa42-62.Thereappeartobetwosplicevariants.Oneshowsafouraadeletionofaa59-62andasecondshowsanalternatestartsiteatMet379.Overaa160-349,humanADPNshares61%aaidentitywithmouseAdiponutrin.
Image:ADPNsatiningofhumanadipocytes(dilution:10µg/mL).Cellswerestainedusing557donkeyanti-sheepIgGsecondaryantibody(red)andcounterstainedwithDAPI(blue).