Type:GoatIgG
Applications:IHC;WB;E
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:R
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:AffinityPurified-liquid
Immunogen:Recombinantrattumornecrosisfactoralpha
Image:TNF-alphaincryostattissuesectionsofratspinalcord.
Tumornecrosisfactorreceptor1and2(TNF-R1andTNF-R2),are55and75kDamembersofafamilycellsurfacemoleculesincludingnervegrowthfactorreceptor,Fas/Apo-1,CD30,Ox40and4-1BB,whicharecharacterizedbycysteinerichmotifsintheextracellulardomain.WhileTNF-R1andTNF-R2share28%sequenceidentityintheirextracellulardomains,theirintracellulardomainslacksequencehomologywhichsuggeststhattheydifferintheirinternalsignaltransductionpathways.TNF-R1initiatesthemajorityofTNF.sactivities,whichincludestriggeringapoptosisofcertaintumorcells,mediatingtheinflammatoryresponseandregulatingimmunefunction(Chen).EachpathwayischaracterizedbyTNF-inducedformationofamulti-proteinsignalingcomplexatthecellmembrane.TNFandTNF-R1bindinginitiatesaseriesofintracellulareventsthatresultinactivationofthetranscriptionfactorsNFkappaBandc-Jun.TNFandTNF-R1bindingtriggersreleaseofthe80residueinhibitoryproteinsilencerofdeathdomains(SODD)fromtheC-terminalintracellulardomainofTNF-R1.ThisdomaininteractswithTNFreceptor-associateddeathdomain(TRADD)whichinturnrecruitsreceptor-interactingprotein(RIP)TNF-R-associatedfactor2(TRAF2)andFas-associateddeathdomain(FADD).ThesethreeproteinsrecruitenzymesresponsIBLeforinitiatingsignalingevents.Forexample,interactionofSODDwithTRADD,andsubsequentinteractionswithFADDinitiatesCaspase-8mediatedapoptosis.TRAF2linksTNF-R1withstressactivatedproteinkinase(SAPK)andp38mitogenactivatedproteinkinasesviagerminalcenterkinase(GCK)andreceptorinteractingprotein(RIP).ThecytoplasmicdomainofTNF-R1candirectlyinteractwithJakkinases.
TNF-alphaCustomerPublications