
Type:MouseIgG
Applications:FC;NB
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:M
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:ProteinGPurified-liquid
Immunogen:HybridomaresultingfromthefusionofamousemyelomawithBcellsobtainedfromaratimmunizedwithmouseCXCR2-transfectedC6cells.
Chemokinereceptorsareseven-transmembranedomainG-proteincoupledreceptorsthatmediatetheBIOLOGicalactivitiesofchemokines.Mostofthesereceptorsexhibitpromiscuousbindingpropertieswherebyseveraldifferentchemokinessignalthroughthesamereceptor.Theyarenamedaccordingtothechemokinesubfamilytheybind.TherearecurrentlysixCXC-specificreceptorsdesignatedCXCR1toCXCR6,elevenCC-specificreceptorsdesignatedCCR1toCCR11,andoneCreceptor,XCR1.CX3CR1isthereceptorforfractalkine.
NeutralizationofHumanCellSurfaceCXCR2mediatedBioactivity-TheexactconcentrationofantibodyrequiredtoneutralizethemousecellsurfaceCXCR2mediatedbioactivityisdependentontheconcentrationaswellasonthenumberofCXCR2receptorspresentonthecellsurface(afunctionofcelltypeandcultureconditions).Toprovideaguideline,R&DSystemshasdeterminedtheneutralizationdoseforthisantibodyunderaspecificsetofconditions.TheNeutralizationDose50(ND50)forthisantibodyisdefinedasthatconcentrationofantibodyrequiredtoyieldone-halfmaximalinhibitionofthecellsurfaceCXCR2mediatedrmMIP-2responseonresponsivecellsataspecificrmMIP-2concentration.
TheND50forthislotofanti-mouseCXCR2antibodywasdeterminedtobeapproximately15-50µg/mLinthepresenceof2ng/mLrmMIP-2inachemotaxisassayusingBaF/3cellstransfectedwithmCXCR2.Thespecificconditionsaredescribedinthefigurelegends.