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Neuromics/CXCR2/IL-8 RB-Fluorescein Labeled/FC15002/100 Tests

Type:MouseIgG
Applications:FC
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:H;R
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:ProteinGPurified-liquid
Immunogen:Clone#:48311

ThehumanC-X-CchemokineIL-8isapotentneutrophilchemotacticandactivatingfactor(1).TwodistinctcellsurfacereceptorscaninteractwiththeIL-8molecule.ThesetwostructuresareknownasIL-8RA(typeIorCXCR-1)andIL-8RB(typeIIorCXCR-2)(2,3).Thetworeceptorsshare77%aminoacidhomologyandarebothmembersoftherhodopsinsuperfamilyofGprotein-linkedreceptorsthatspanthecellmembranewithsevenhydrophobictransmembranedomains.OfthetwoIL-8receptors,onlyIL-8RAexclusivelybindsIL-8(4).AthirdIL-8bindingstructure,knownastheDuffybloodgroupantigen,hasbeenidentifiedonredbloodcells(5).IL-8RAexpressionhasbeendocumentedonneutrophils,monocytesandasmallpopulationofTcells(2).TheCXCR-2receptorhastheABIlitytobindavarietyofchemokines,includingIL-8,GROα,βandγ,NAP-2andENA-78.
Images:A.TypicalstainingobservedwithFAB331FonmousecellstransfectedwiththehumanCXCR-1,CXCR-2orCXCR-4gene.AnisotypecontrolstainingoftheCXCR-2transfectedcellsisalsoshown.B.TypicalstainingobservedwithFAB331Fonhumanperipheralgranulocytes.Anisotype-matchedcontrolstainingofthesamegranulocytesisalsoshown.