
Type:MouseIgG
Applications:FC
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:H
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:ProteinGPurified-liquid
Immunogen:Clone#:44717
CXCR-4(fusinorLESTR)isaG-proteinlinkedseventransmembranespanningchemokinereceptorthatbindsstromalcellderivedfactor-1(SDF-1)(1,2).Additionally,CXCR-4hasbeenfoundtoactasaco-factorfortheT-celltropicHIV-1and-2virusentryintocells(3,4).Amonoclonalantibody(clone12G5)totheCXCR-4structurewasdevelopedbyEndersetal.(4)andshownbyflowcytometryapplicationstoreactwithCXCR-4expressedonavarietyofhumancelllines,includingSup-T1,Hut-78,Molt4,CEMss,DaudiandHela,aswellaswithperipheralbloodlymphocytes(4).Clone12G5canneutralizeinfectionandinhibitsyncytiumformationinducedbytheHIVvirus(4).Theepitopeforthe12G5monoclonalantibodymapstoaregionoftheCXCR-4receptorthatincludesextracellularloops1and2,withthefirstextracellularloopplayingamorecriticalroleintheepitopestructure(5).R&DSystemshasdevelopedaseriesofmonoclonalantibodiesthatspecificallyreactwiththehumanCXCR-4structureontransfectedcellsaswellastheCXCR-4antigenonbothstimulatedandunstimulatedperipheralbloodlymphocytes.Clone44717.111appearstoreactpreferentiallywiththesecondexternalloopregionoftheCXCR-4receptor.CXCR4CustomerPublications.