Type:GoatIgG
Applications:E;ICC;WB;NB
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:R
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:AffinityPurified-liquid
Immunogen:purified,E.coli-derived,recombinantratinterleukin1beta.
TheInterleukin1receptorfamily(IL-1R)comprisesatleastninemembersincludingIL-1R1,IL-1R2(IL-1RII),IL-1R3(IL-1RAcP),IL-1R4(ST2/T1),IL-1R5(IL-18Ralpha/IL-1Rrp),IL-1R6(IL-1Rrp2/IL-1RL2),IL-1R7(AcPL/IL-18Rb),IL-1R8(TIGIRR-2)andIL-1R9(TIGIRR-1).Allfamilymemberspossessthreeimmunoglobulin(Ig)-likedomainsintheirextracellularregion.MostmembersalsohaveanintracellularTIR(Toll-likereceptor/IL-1receptor)signalingdomainthatisalsoconservedintheToll-likereceptorfamily.AsubtypeoftheIL-1Rfamily,SIGIRR(singleIgdomain-containingIL-1R-relatedmolecule)whichdiffersfromtheotherninemembersbyhavingonlyoneIgdomain,hasalsobeenidentified.SeeIL-18RalphaforIL-1R5andIL-18RbetaforIL-1R7.
Image:IL-1betawasdetectedinLPS-stimulatedratsplenocytes.Cellswerestainedusinganti-goatIgGsecondaryantibody(red)andcounterstainedwithFluoroNissl™*Green.
*FluoroNisslisatrademarkofMolecularprobes.
![Picture[2]](images/neuromics/2017/A8x5c72x66x1.jpg)
Figure1:RatIL-1βstimulates3H-thymidineincorporationbymurineT-helperD10.G4.1cellsinadose-dependentmanner(Symons,J.A.etal.,1987,inLymphokinesandInterferons,APracticalApproach,IRLPress,M.J.Clemens,A.G.MorrisandA.J.H.Gearing,eds.p.272).TheED50forthiseffectistypically1-3ng/mL.
Figure2:Approximately0.5-2.0μg/mLoftheantibodywillneutralize50%ofthebioactivitydueto10ng/mLofratIL-1β.TheND50forthislotofanti-ratIL-1βantibodywasdeterminedtobeapproximately0.5-2μg/mLinthepresenceof10ng/mLofrrIL-1β,usingthemurineT-helpercellline,D10.G4.1.Thespecificconditionsaredescribedinthefigurelegends.
