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Neuromics/Macrophage Inflammatory Protein 1alpha (MIP-1a)/CCL3/GT15070/100 ug

Type:GoatIgG
Applications:IHC;WB;E
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:H
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:AffinityPurified-liquid
Immunogen:RecombinanthumanMIP-1alpha.

MIP-1alpha(macrophageinflammatoryprotein1alpha)isamemberoftheCCorbetachemokinesubfamilythatwasoriginallypurifiedfromtheconditionedmediaofanLPS-stimulatedmurinemacrophagecellline.MIP-1alphaactsasachemoattractanttoavarietyofcellsincludingmonocytes,Tcells,Bcellsandeosinophils.ThetwohumanMIP-1alphagenesarisebyduplication/mutation.TheycodeforMIP-1alphaisoformsCCL3/LD78aandCCL3L1/LD78b,whichshare94%aminoacidsequencehomology.WhereasthehumanCCL3/LD78aisasingle-copygene,thehumanCCL3L1/LD78bgenecopynumbervarieswithinthepopulation.HumanCCL3L1/LD78bbindsandsignalsthroughchemokinereceptorsCCR1,CCR5.WhencomparedtoCCL3/LD78a,CCL3L1/LD78bhashigherbindingaffinitytoCCR5,whichalsofunctionsasacoreceptorforHIV-1entry.ThecopynumberofCCL3L1isoneofseveralgeneticdeterminantsofHIV-1susceptibility.
Image:Paraffin-embeddedtissuesectionsofhumanhippocampus(Alzheimer"s).MIP-1alphaimmunoreactivityisshowninredandHaematoxylincounterstaininblue.