
Type:MouseIgG
Applications:IF;WB
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:B;H;M;P;R
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:Supernatant-liquid
Immunogen:RecombinantfulllengthhumanDoublecortinpurifiedfromE.coli.

Doublecortin(DCX)hasamolecularweightofabout45kDa.Theproteincontainstwodomains,eachabout90aminoacidslong,whicharebelievedtofunctioninbindingtomicrotubules,aC-terminalserineandprolinerichregionwhichmaybecomephosphorylatedinvivo.DCXisexpressedveryearlyinneuronaldevelopment,asneuroblastsbecomepost-mitotic,butislostasneuronsmature.DevelopingneuronsstarttoloseDCXexpressionaboutthetimethattheybegintoexpressNeuN,aneuronalspecificRNAbindingproteincharacteristicofmorematureneurons.AntibodiestoDCXareusedtoidentifystemcellsinsectionsandintissueculture,andtoseeifneurogenesisistakingplace.
Image:E18hippocampalneuronsstainedwithMAPT(red)andDoublecortin(green).Thetwoproteinsoverlapintheproximaldendrites,butdoublecortinismoreabundantinthegrowthconesandperiphery.Asaresult,theperipheryappearsgreenwhilethemoreproximalregionsofthecellsareyellow.Thesinglelongerprocessofthiscell,presumablyanaxon,hasalowdoublecortincontentandsoappearsred.BluestainingisthenuclearDNA. Protocolondatasheet.
DefectsinDCXarethecauseoflissencephalyX-linkedtype1(LISX1)[MIM:300067];alsocalledX-LISorLIS.LISX1isaclassiclissencephalycharacterizedbymentalretardationandseizuresthataremoresevereinmalepatients.