Type:RabbitIgG
Applications:IHC;WB
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:B;H;M;R
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:Wholeserum-liquid
Immunogen:RecombinantProtein
GlutamineSynthetase,alsocalledglutamate-ammonialigase(GLUL),isexpressedthroughoutthebodyandplaysanimportantroleincontrollingbodypHandinremovingammoniafromthecirculation.TheenzymeclearsL-glutamate,themajorneurotransmitterinthecentralnervoussystem,fromneuronalsynapses.
Images:Localizationofglutaminesynthaseintheretina.Paraffinsectionsofmouse(A,B),rat(C,D,G-I),orhuman(E,F)retinafixedin4%paraformaldehydewerereactedwithanti-glutaminesynthase(redfluorescencestaininginB,D,G,I,andbrownimmuno-peroxidasereaction[usingABCkitandvisualizationwithDABproductinF].Nucleiinsomeimmunofluorescenceexperiments(A-D)werestainedwithDAPI(shownincyan),andwithnuclearfastredinEandF.Oninspectionatlowmagnification,anti-glutaminesynthasereactedwithasinglepopulationofcellsextendingfromtheganglioncelllayer(GCL)throughtheinnernuclearlayer(INL).Nosignalwasdetectedincontrolseitherpre-incubatedwith100ug/mloftheimmunizingpeptide(A)orwithpre-immuneserum(C,E).ThepatternofstainingobservedinallexperimentsistypicalofMüllercells.Thisfindingwasconfirmedbyco-localization(indicatedbyyellowinI)ofglutaminesynthase(redinG)withantoherMarkerofglutaminesynthase(greeninH).
ItsactivityisdecreasedinthecerebralcortexofbrainsaffectedbyAlzheimer"sdisease,particularlyinthevicinityofsenileplaques.Itisalsodecreasedunderconditionsofglucosedeprivation.ThelevelofexpressionofGlutamineSynthetaseisincreasedduringischemiainvivoorhypoxiainculture.
