Type:MouseIgG
Applications:IF;WB
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:B;H;M;P;R
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:AffinityPurified-liquid
Immunogen:PurifiedfulllengthrecombinanthumanLaminC,andreactswithbothLaminAandC.
Laminsfunctionasskeletalcomponentstightlyassociatedwiththeinnernuclearmembrane.OriginallytheproteinsofthenuclearCytoskeletonwerenamedLaminA,BandC,fromtoptobottomasvisualizedonSDS-PAGEgels.SubsequentlyitwasfoundthatLaminsAandCwerecodedforbyasinglegene,whiletheLaminBbandmaycontaintwoproteinsencodedbytwogenesnowcalledLaminB1andLaminB2.LaminAhasamassofabout74kDawhileLaminCis65kDa.TheLaminAproteinincludes98aminoacidsmissingfromLaminC,whileLaminChasaC-terminal6aminoacidpeptidenotpresentinLaminA.ApartfromtheseregionsLaminAandCareidenticalsothatantibodiesraisedagainsteitherproteinarelikelytocrossreactwiththeother,asisthecasewiththismonoclonal.
Image:HeLacellsstaingwithLaminA/C(red),andcounterstainedwithchickenpolyclonalVimentinantibodyto(green)andDNA(blue).Theantibodyrevealsstrongnuclearlaminastaining,whiletheVimentinantibodyrevealscytoplasmicintermediatefilaments.Protocolondatasheet.
ActivityofthiskinaseincreasesduringcelldivisionandisresponsIBLeforthebreakdownofthenuclearlamina.MutationsintheLMNAgeneareassociatedwithseveralserioushumandiseases,includingEmery-Dreifussmusculardystrophy,familialpartiallipodystrophy,limbgirdlemusculardystrophy,dilatedcardiomyopathy,Charcot-Marie-Toothdiseasetype2B1,andHutchinson-Gilfordprogeriasyndrome.ThisfamilyofdiseasesbelongtoalargergroupwhichareoftenreferredtoasLaminopathies,thoughsomelaminopathiesareassociatedindefectsinLaminB1,B2oroneorotherofthenumerousnuclearlaminabindingproteins.AtruncatedversionoflaminA,commonlyknownasprogerin,causesHutchinson-Gilfordprogeriasyndrome,aformofprematureaging.
