
Type:MouseIgG
Applications:ICC;IF;IHC
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:M
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:AffinityPurified-liquid
Immunogen:KLHconjugatedsyntheticphosphopeptidecorrespondingtoaminoacidresiduessurroundingS421ofhumanMeCP2.

Methyl-CpGBindingProtein2(MeCP2)isanuclearproteinthatisfoundtobindtosymmetricalmethylatedCpGdinucleotidesequences.ItisatranscriptionalmodulatorthatcanaltergeneexpressionepigeneticallyviabindingtomethylatedDNA. Itisinvolvednotonlyintranscriptionalsilencing,butalsointranscriptionalactivation,chromatinremodeling,andRNAsplicing. Mecp2isaX-chromosome-linkedgene,highlyexpressedinneurons.Themutationsof Mecp2 arelinkedtoRettsyndrome(RTT),whichisaneurodevelopmental,autisticdisorderthataffectsmainlyfemales.Studies showthateventhelossofaspecificphosphorylationsiteof MeCP2 (e.g.,S80,S421,andS424)disturbsnormalmaturationofthemammalianbrain.
Image:Mousebrainsection,whichwascutat45μMonavibratomeafterperfusionwith4%parformaldehydeandovernightfixation,was stainedwithmonoclonalantibodyat1:1,000:MO22150.Phospho-MeCP2atS421wasmainlyassociatedwith methylatedDNA in nucleusof activatedneuroncells (red).BlueshowsDAPIstainingofnuclearDNA.