
Type:MouseIgG
Applications:ICC;IF;IHC;WB
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:B;Ch;H;M;P;R
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:AffinityPurified-liquid
Immunogen:Full-lengthrecombinanthumanMuscleblind-like1protein

Muscleblind-likeProtein(MBNL1) mediatespre-mRNAalternativesplicingregulation.Actseitherasactivatororrepressorofsplicingonspecificpre-mRNAtargets.Inhibitscardiactroponin-T(TNNT2)pre-mRNAexoninclusionbutinducesinsulinreceptor(IR)pre-mRNAexoninclusioninmuscle.AntagonizesthealternativesplicingactivitypatternofCELFproteins.RegulatestheTNNT2exon5skippingthroughcompetitionwithU2AF2.InhibitstheformationofthespliceosomeAcomplexonintron4ofTNNT2pre-mRNA.Bindstothestem-loopstructurewithinthepolypyrimidinetractofTNNT2intron4duringspliceosomeassembly.Bindstothe5-YGCU(U/G)Y-3consensussequence.BindstotheIRRNA.BindstoexpandedCUGrepeatRNA,whichfoldsintoahairpinstructurecontainingGCbasepairsandbulged,unpairedUresidues.
Image:HeLacellswerestainedwithmonoclonalantibodyMuscleblind-likeprotein(MO22147)at1:1,000.Muscleblind-likeprotein1ispresentinthenucleusofHeLacells(red).Co-stainingiswithourrabbitpolyclonalantibodyagainstvimentin(RA22124,inred)revealscytoplasmicintermediatefilaments. BlueisaDNAstainwhichlargelycolocalizeswiththeMBNL1proteininthenucleus.