
Type:MouseIgG
Applications:ICC;IHC;WB
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:Ch;H;M;Pr;R
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllImmunogen:NativeNF-Hpurifiedfrompigspinalcord

Neurofilamentscanbedefinedastheintermediateor10nmfilamentsfoundinspecificallyinneuronalcells.Intheelectronmicroscopeneurofilamentsappearsas10nmdiameterfibresofindeterminatelengthwhichgenerallyhavefinewispyprotrusionsfromtheirsides.Theyarefoundparticularlyabundantlyinaxonsoflargeprojectionneurons.AntibodiestothevariousneurofilamentsubunitsareveryusefulcelltypeMarkerssincetheproteinsareamongthemostabundantofthenervoussystem,areexpressedonlyinneurons,andarebiochemicallyverystable.
Image:Mixedneuron/glialculturesstainedwithMO22103(red)andrabbitGFAPantibody(green).Axonalprofilesarestainedinred,whileastrocyticcellsarerevealedingreen.NucleiarerevealedwithafluorescentDNAstain(blue).
ThisantibodyrecognizesphosphorylatedNF-HKSP(lysine-serine-proline)typesequences.Insomespeciesthereissomecross-reactivitywiththerelatedphosphorylatedKSPsequencesfoundintherelatedneurofilamentsubunitNF-M.TheantibodyrecognizesNF-Hstronglyinallmammalstestedtodateandalsoinchicken.Itrecognizesneurofilamentsinfrozensectionsintissuecultureandinformalinfixedsections