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Neuromics/Neurofilament NF-H, phosphate independent/RA22112/100 ul
Type:RabbitIgG
Applications:IF;WB
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:R
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:Wholeserum-liquid
Immunogen:KSPregionofratNF-HwhichwasexpressedinandpurifiedfromE.coli.
Neurofilamentscanbedefinedastheintermediateor10nmfilamentsfoundinspecificallyinneuronalcells.Intheelectronmicroscopeneurofilamentsappearsas10nmdiameterfibresofindeterminatelengthwhichgenerallyhavefinewispyprotrusionsfromtheirsides.Theyarefoundparticularlyabundantlyinaxonsoflargeprojectionneurons.AntibodiestothevariousneurofilamentsubunitsareveryusefulcelltypeMarkerssincetheproteinsareamongthemostabundantofthenervoussystem,areexpressedonlyinneurons,andarebiochemicallyverystable.
Images:Mixedneuron/glialratbrainculturesstainedwithRPCA-NF-H-pind(green)andchickenpolyclonalantibodytoGlialFibrillaryAcidicProtein-GFAP (red).TheneurofilamentNF-HantibodybindstophosphorylatedaxonalformsofNF-Handnon-phosphorylateddendriticandperikaryalforms.Protocolondatasheet.
MammalianNF-Hcontainstandemrepeatsbasedonthesequencelysineserineproline(KSP),whichmaybepresentupto60times,withsomevariABIlitybetweenspecies.TheseKSPsequencesarethesitesofphosphorylation,andinaxonsmostifnotalloftheserineresiduesarephosphorylated.Incontrast,neurofilamentsindendritesandperikaryaarenotphosphorylatedontheseKSPsequences.Axonalneurofilamentsarethereforequitedifferentimmunologicallyfromthoseinperikaryaanddendrites,andmanyantibodiesstainonlyaxonaloronlydendritic/perikaryalneurofilaments.Sincebacteriadonotphosphorylatemammalianproteins,thisproteinresemblestheNF-Hformcharacteristicofperikaryalanddendriticneurofilaments.Accordinglythisantibodystainsdendriticandperikaryalneurofilamentsparticularlywell.
