
Type:RabbitIgG
Applications:IHC;WB
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:H;Pr;R
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:AffinityPurified-liquid
Immunogen:ApeptidederivedfromthehumanOgg1(withinaminoacids1-100).

8-hydroxyguanine,aformofoxidativeDNAdamageinducedbyfreerADIcals,causesG:CtoT:Atransversion.InE.coli,threeDNArepairenzymesexisttopreventthemutageniceffectsof8-hydroxyguanine.Oneoftheseenzymes,MutM,wasfoundtohaveafunctionalyeast(yOgg1)andhuman(hOgg1)homologue.hOgg1proteinsefficientlyreleasethe8-hydroxyguanineoppositethepyrimidinefromDNAandcleavetheAPsiteinamannersimilartobacterialandyeastenzymes.GeneticbackgroundsincontroloftherepairofdamagedDNAareinvolvedinthesusceptibilitytocancerdevelopment.ThehOgg1genehasbeenmappedtoregion3p26.2,aregionshowinglossofheterozygosity(LOH)inavarietyofcancers.Inparticular,3p25-p26isacommonLOHregioninlungcancer.RecentworkhasdemonstratedthatOggplaysanimportantroleinCAGexpansion,acharacteristicofseveralneurodegenerativediseases.OggappearstoberesponsIBLeforprogressiveexpansionofpoly-Qtractsinresponsetooxidativedamage.Thus,OggprovidesadirectlinkbetweenDNAdamageandtoxicityinneurons.
Image:Ogg1satining offormaldehydefixedfrozensectionsofthesubstantianigrafromaRhesusmacaque(Macacamulatta).PhotocourtesyofGlenKisby,OregonHealthSciencesUniversity.