Type:MouseIgG
Applications:IF;WB
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:H;M;R
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:AscitesFluid-liquid
Immunogen:Epitopefor?-Synucleinisintheregion61-95whichcorrespondtothe"Non-AmyloidbetaComponentofAlzheimer"sdiseaseamyloid"
Note:Thisantibodywillalsobindhumanalpha-synucleincontainingtheA30PandA53Tmutations.
Alphasynucleinisamemberofthesynucleinfamily,theothertwoproteinsbeingbetaandgammasynuclein.AlphasynucleinwasoriginallyisolatedasamajorsynapticvesicleassociatedproteinfromtheelectricorganofthefishTorpedo(1).Directhomologuesofalpha-synucleinarefoundinallvertebrates.Laterworkconnectedalpha-synucleinwithhumanbrainpathology,whenaproteinoriginallyidentifiedasacomponentofNAC,the"Non-AmyloidbetaComponentofAlzheimer"sdiseaseamyloid"provedtobeapeptidederivedfromalpha-synuclein(2).
Image:Mixedneuron-glialculturesstainedwithalpha-synuclein (red)andchickenpolylclonalantibodytoMAP2 (green).Thealpha-synucleinantibodystainsvesicularstructurestheperikaryaandprocessesoftheneuronsinthis Notethatsomeoftheneuronalperikaryacontainmuchmorealpha-synucleinthanothers.ThebluechannelshowsthelocalizationofDNA.ProtocolonData-sheet.
Thealpha-synucleinproteinisthereforesometimesknownasNACprecursororNACP.Furtherworkshowedthatalpha-synucleinisamajorcomponentoftheLewybodiesofParkinson"sdiseaseandpointmutationsofalpha-synucleinprovedtobecausativeofsomeformsoffamilialParkinson"sdisease(3,4,5).However,despitebeingdiscoveredasascomponentofamyloidpreparations,alpha-synucleinisapparentlynotamajorcomponentofthesenileplaquesofAlzheimer"sdisease(6).EarlyonsetParkinson"sdiseasemaybecausedbyaduplicationortriplicationofoneofthealphasynucleingenes(7,8).Alpha-synucleinisalsofoundintheLewybodiesofpatientswithdiffuseLewybodydiseaseandinclusionsinglialcellsinthebrainsofpatientswithmultiplesystematrophy(MSA)andamyotrophiclateralsclerosis(ALS).Alpha-synucleinisheavilyexpressedinbrainandappearstobelocalizedprimarilytopresynapticregions,thoughnotwithatypicalsynapticvesicledistributionpattern.Thesynucleinproteinsappeartohavelittle3Dstructureinsolution,andprobablybelongtothefamilyof"intrinsicallyunstructuredproteins"whichonlyadoptawell-definedconformationwhenboundtootherproteinsormembranelipids(9).Anexcellentrecentreviewoftheroleofalpha-synucleininhealthanddiseasewasrecentlypublishedbyMarkCookson(10).
